By Charles Dickens
One of the best-loved and such a lot quoted tales of "the guy who invented Christmas"-English author Charles Dickens-A Christmas Carol debuted in 1843 and has touched thousands of hearts since.
Cruel miser Ebeneezer Scrooge hasn't ever met a shilling he doesn't like. . .and rarely a guy he does. And he hates Christmas such a lot of all. while Scrooge is visited via his outdated companion, Jacob Marley, and the ghosts of Christmas earlier, Christmas current, and yuletide but to return, he learns everlasting classes of charity, kindness, and goodwill. event a real Victorian Christmas!
A miser learns the real that means of Christmas whilst 3 ghostly viewers evaluation his earlier and foretell his destiny.
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Construction of the plasmid is described in the text. This cloning vehicle is suitable for use with the insertional activation procedure for EcoRI, Hindlll, BamI, or Sali DNA fragments. In addition, it is a non-conjugative, multicopy cloning vector that is amplifiable in the presence of appropriate concentrations of chloramphenicol. A wide variety of other methods have been used to enable selection of plasmids carrying specific DNA fragments; in general, these involve the use of radioactively labelled DNA or RNA probes or immunological probes to identify clones carrying the desired chimeric plasmids or phage.
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